5XEA
Structure of Thogoto virus envelope glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.198, 66.768, 120.737 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 35.325 - 2.093 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3duz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmerge | 0.089 | 0.794 |
| Number of reflections | 95874 | 8850 |
| <I/σ(I)> | 16.832 | |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 10.000 |
