5XE0
Crystal structure of EV-D68-3Dpol in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-25 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.093, 81.436, 58.446 |
| Unit cell angles | 90.00, 108.66, 90.00 |
Refinement procedure
| Resolution | 26.210 - 2.300 |
| R-factor | 0.2258 |
| Rwork | 0.224 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n6m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.564 |
| Data reduction software | CrysalisPro (v.1.171.33.49) |
| Data scaling software | CrysalisPro (v.1.171.33.49) |
| Phasing software | PHENIX (1.10.1) |
| Refinement software | REFMAC (ccp4 6.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.210 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.201 | 0.939 |
| Number of reflections | 21405 | 3156 |
| <I/σ(I)> | 6 | 1.1 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289.15 | 0.1 M Tris-HCl pH 8.5, 20.1% isopropanol, 20.1% PEG 3350 |
