5XDW
Staphylococcus aureus FtsZ 12-316 G196S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.764, 51.011, 88.328 |
| Unit cell angles | 90.00, 110.71, 90.00 |
Refinement procedure
| Resolution | 41.310 - 2.000 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3voa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.448 |
| Data reduction software | HKL |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.146 | 0.050 | 0.879 |
| Rmeas | 0.162 | 0.056 | 0.967 |
| Rpim | 0.068 | 0.025 | 0.400 |
| Total number of observations | 110946 | ||
| Number of reflections | 20347 | ||
| <I/σ(I)> | 6.1 | ||
| Completeness [%] | 99.7 | 98.7 | 99.8 |
| Redundancy | 5.5 | 4.8 | 5.7 |
| CC(1/2) | 0.998 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM Tris-HCl, 45% w/v PEP629, 300mM KCl |
