5XDU
Staphylococcus aureus FtsZ 12-316 complexed with TXA6101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.759, 51.574, 86.661 |
| Unit cell angles | 90.00, 108.75, 90.00 |
Refinement procedure
| Resolution | 40.900 - 2.000 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3voa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.081 | 0.063 | 0.264 |
| Rmeas | 0.099 | 0.075 | 0.322 |
| Rpim | 0.055 | 0.040 | 0.182 |
| Total number of observations | 55580 | ||
| Number of reflections | 19640 | ||
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 97.7 | 99.6 | 98.3 |
| Redundancy | 2.8 | 3.4 | 2.7 |
| CC(1/2) | 0.991 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 100mM Tris-HCl, 43% w/v PEP629, 300mM KCl |
