5XDP
K11/48-branched teraubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-08 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.938, 26.163, 46.540 |
| Unit cell angles | 103.03, 94.16, 99.65 |
Refinement procedure
| Resolution | 25.402 - 2.376 |
| R-factor | 0.2362 |
| Rwork | 0.234 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.764 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.410 |
| High resolution limit [Å] | 2.376 |
| Number of reflections | 5120 |
| <I/σ(I)> | 1.28 |
| Completeness [%] | 84.0 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 289 | 0.2M Potassium nitrate, 20% w/v Polyethylene glycol 3350 |
