5XDC
Crystal structure of Indole-bound TdsC from Paenibacillus sp. A11-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-21 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.830, 100.830, 424.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.491 - 1.579 |
| R-factor | 0.1658 |
| Rwork | 0.165 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.791 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.670 |
| High resolution limit [Å] | 1.579 | 1.580 |
| Rmerge | 0.629 | |
| Number of reflections | 509113 | 59173 |
| <I/σ(I)> | 9.9 | 1.23 |
| Completeness [%] | 99.9 | 63.7 |
| Redundancy | 3.9 | 1.86 |
| CC(1/2) | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.4 M ammonium sulfate, 0.1 M Tris-HCl, 12% (v/v) Glycerol |
