5XD7
Crystal structure analysis of 3,6-anhydro-L-galactonate cycloisomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 87.336, 87.336, 143.069 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.679 - 2.198 |
R-factor | 0.1911 |
Rwork | 0.188 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ovl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.064 |
Data reduction software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.198 |
Number of reflections | 430401 |
<I/σ(I)> | 23.4 |
Completeness [%] | 99.7 |
Redundancy | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate (pH 6.5), and 20% (w/v) polyethylene glycol 8,000 |