5XD6

CARK1 phosphorylates ABA receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.715, 46.661, 110.512
Unit cell angles	90.00, 105.77, 90.00

Refinement procedure

Resolution	29.755 - 1.898
R-factor	0.2185
Rwork	0.217
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.898	1.900
Rmerge	0.055	0.596
Number of reflections	53775	2522
<I/σ(I)>	15	1.94
Completeness [%]	98.4	93.2
Redundancy	3.9	3.1
CC(1/2)	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M ammonium acetate, 0.1M Bis-Tris (pH 5.5), 17%(w/v) PEG 10,000