5XCV
Crystal structure of NZ-1 Fv-clasp fragment with its antigen peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.617, 50.122, 121.868 |
| Unit cell angles | 90.00, 110.46, 90.00 |
Refinement procedure
| Resolution | 41.762 - 2.143 |
| R-factor | 0.2307 |
| Rwork | 0.228 |
| R-free | 0.27660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xct 4yo0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2341: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 46235 | |
| <I/σ(I)> | 16.05 | 3.39 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M Calcium acetate, 0.1M MES (pH 6.5), 20%(w/v) PEG8000 |
