5XCR
Crystal structure of P20.1 Fv-clasp fragment with its antigen peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-04 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.060, 80.660, 85.700 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 46.660 - 1.750 |
| R-factor | 0.17235 |
| Rwork | 0.171 |
| R-free | 0.20224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xcq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.548 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 75053 | |
| <I/σ(I)> | 16.69 | 3.22 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 7.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Citrate (pH 5.5), 20%(w/v) PEG3000 |
