5XCK

Crystal structure of Vps29 double mutant (D62A/H86A) from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.95
Spacegroup name	P 1 21 1
Unit cell lengths	35.070, 67.441, 77.614
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	33.636 - 2.200
R-factor	0.2495
Rwork	0.247
R-free	0.27880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xce
RMSD bond length	0.003
RMSD bond angle	0.643
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	17278
<I/σ(I)>	14
Completeness [%]	93.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	200 mM Sodium Acetate, 100mM Sodium cacodylate pH 6.5, 30% PEG 8K