5XCJ
Crystal structure of Vps29 single mutant (D62A) from Entamoeba histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.428, 67.364, 77.931 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.729 - 1.859 |
R-factor | 0.2108 |
Rwork | 0.206 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xce |
RMSD bond length | 0.007 |
RMSD bond angle | 1.305 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.850 |
Number of reflections | 30853 |
<I/σ(I)> | 8.5 |
Completeness [%] | 100.0 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 200 mM Sodium Acetate, 100mM Sodium cacodylate pH 6.5, 30% PEG 8K |