5XCH
Crystal structure of Wild type Vps29 complexed with Zn+2 from Entamoeba histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-23 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.2 |
Spacegroup name | P 32 |
Unit cell lengths | 47.310, 47.310, 148.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.900 - 2.850 |
R-factor | 0.2879 |
Rwork | 0.287 |
R-free | 0.30680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xce |
RMSD bond length | 0.005 |
RMSD bond angle | 0.842 |
Data reduction software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.900 |
High resolution limit [Å] | 2.850 |
Number of reflections | 8561 |
<I/σ(I)> | 21 |
Completeness [%] | 98.0 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 10 % w/v PEG 20000, 20 % v/v PEGMME 550, 0.02 M of alcohol MIX, 0.1 M MES/Imidazole pH 6.5 |