5XCC

X-ray structure of Clostridium perfringens pili protein CppA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	63.340, 71.330, 228.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	114.380 - 2.480
R-factor	0.22974
Rwork	0.228
R-free	0.27182
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xcb
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.380	2.540
High resolution limit [Å]	2.480	2.480
Rmerge	0.068	0.490
Number of reflections	37465
<I/σ(I)>	16.08	3.71
Completeness [%]	99.4	98.6
Redundancy	5.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Sodium formate, 0.1M Ammonium acetate, 0.1M Sodium citrate tribasic dihydrate, 0.1M Sodium potassium tartrate tetrahydrate, 0.1M Sodium oxamate, 0.1M Imidazole, o.1M MES monohydrate (acid), 20% v/v Ethylene glycol, 10% w/v PEG 8000