5XC7

Dengue Virus 4 NS3 Helicase D290A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-04-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.045, 92.172, 102.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.431 - 2.100
R-factor	0.1912
Rwork	0.189
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jlq
RMSD bond length	0.011
RMSD bond angle	1.108
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.431
High resolution limit [Å]	2.100
Number of reflections	30062
<I/σ(I)>	15.88
Completeness [%]	100.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	286	0.1M MES, 20% polyethylene glycol 3350