5XC3

Crystal structure of Acanthamoeba polyphaga mimivirus Rab GTPase in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	35.575, 66.726, 69.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.691 - 1.497
R-factor	0.1807
Rwork	0.179
R-free	0.19920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tu4
RMSD bond length	0.005
RMSD bond angle	0.938
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.497
Number of reflections	27374
<I/σ(I)>	51.9
Completeness [%]	99.7
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M sodium iodide and 20% (w/v) polyethylene glycol 3350