5XBY

Crystal structure of the PKA-Protein A fusion protein (end-to-end fusion)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2017-03-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	75.988, 174.147, 103.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.537 - 3.250
R-factor	0.25359
Rwork	0.251
R-free	0.30675
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2izx 4znc
RMSD bond length	0.016
RMSD bond angle	1.729
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.250	3.300
Number of reflections	10889
<I/σ(I)>	23.6
Completeness [%]	98.9
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	296	100mM Tris pH 8.5, 2.16M Sodium formate