5XBN
crystal structure of Wss1 from saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 |
Unit cell lengths | 75.195, 75.195, 43.739 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.597 - 1.761 |
R-factor | 0.1853 |
Rwork | 0.182 |
R-free | 0.21370 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.022 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.760 |
Number of reflections | 28156 |
<I/σ(I)> | 82.913 |
Completeness [%] | 99.9 |
Redundancy | 21.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20%(w/v) PEG-3500, 8%(v/v) Tacsimate, pH 7.0 |