5XBJ
The structure of the flagellar hook junction protein HAP1 (FlgK) from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.501, 123.552, 92.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.971 - 2.448 |
R-factor | 0.1834 |
Rwork | 0.178 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d4y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.517 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.620 |
High resolution limit [Å] | 2.450 |
Number of reflections | 21573 |
<I/σ(I)> | 12.8 |
Completeness [%] | 99.8 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 30% PEG MME 2000, 0.4M AMMONIUM, ACETATE, 2% MPD, 4% 2-PROPANOL, 5% ETHYLENE GLYCOL, 0.1M SODIUM CITRATE PH 5.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |