5XBF

Crystal Structure of Myo7b C-terminal MyTH4-FERM in complex with USH1C PDZ3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9787
Spacegroup name	P 1 21 1
Unit cell lengths	69.634, 42.537, 118.390
Unit cell angles	90.00, 97.51, 90.00

Refinement procedure

Resolution	35.293 - 1.802
R-factor	0.1883
Rwork	0.187
R-free	0.21070
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	0.997
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.059	0.031	0.492
Rmeas	0.071	0.036	0.595
Rpim	0.039	0.020	0.330
Number of reflections	63640
<I/σ(I)>	11.7
Completeness [%]	99.1	96.6	99.8
Redundancy	3	3	3
CC(1/2)		0.997	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	299	8% tacsimate, pH 7.0 and 20% v/w PEG 3350