5XAQ
Crystal structure of Animalia-specific tRNA deacylase from Mus musculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.379, 75.351, 103.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.940 - 1.860 |
| R-factor | 0.20357 |
| Rwork | 0.201 |
| R-free | 0.24967 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nbi |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.041 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.940 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.076 | 0.380 |
| Number of reflections | 29268 | |
| <I/σ(I)> | 21.8 | 2.4 |
| Completeness [%] | 89.5 | 51.2 |
| Redundancy | 6.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | BICINE (pH 8.0), PEG1500 |
