5XAP
Crystal structure of SecDF in I form (C2 space group)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.265, 69.807, 66.169 |
Unit cell angles | 90.00, 94.86, 90.00 |
Refinement procedure
Resolution | 49.135 - 2.605 |
R-factor | 0.2069 |
Rwork | 0.203 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aqp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.046 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.223 | 0.056 | 0.833 |
Rmeas | 0.234 | 0.059 | 0.904 |
Rpim | 0.072 | 0.018 | 0.340 |
Number of reflections | 28645 | ||
<I/σ(I)> | 4 | ||
Completeness [%] | 99.0 | 99.4 | 98.9 |
Redundancy | 10.2 | 10.9 | 6.5 |
CC(1/2) | 0.999 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.9 | 298 | 41% PEG 200, 100mM Na-citrate, 100mM NH4NO3 |