5XAE
mutNLIR_LC3B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.720, 53.447, 61.584 |
| Unit cell angles | 77.15, 73.18, 85.05 |
Refinement procedure
| Resolution | 39.923 - 1.996 |
| R-factor | 0.2224 |
| Rwork | 0.220 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.727 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.923 |
| High resolution limit [Å] | 1.996 |
| Number of reflections | 32311 |
| <I/σ(I)> | 19.78 |
| Completeness [%] | 95.7 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Pentaerythritol propoxylate, 0.1M HEPES pH 7.0, 0.2M Potassium chloride. |
