5XA7
Complete structure factors and an atomic model of the calcium pump (SERCA1A) and associated phospholipids in the E1-2CA2+ crystals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-13 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.80 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 166.200, 64.545, 146.220 |
Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
Resolution | 82.270 - 3.200 |
R-factor | 0.2263 |
Rwork | 0.225 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1WPE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.550 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 144.750 |
High resolution limit [Å] | 3.200 |
Number of reflections | 25724 |
<I/σ(I)> | 81.76 |
Completeness [%] | 100.0 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | PEG 2000 |