5XA0
Crystal structure of inositol 1,4,5-trisphosphate receptor cytosolic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 42 |
| Unit cell lengths | 128.164, 128.164, 369.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.807 - 5.812 |
| R-factor | 0.2861 |
| Rwork | 0.278 |
| R-free | 0.34170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5X9Z chain A and 3UJ4 chain A |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.779 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 6.020 |
| High resolution limit [Å] | 5.810 | 5.812 |
| Rmerge | 0.570 | |
| Number of reflections | 16347 | 1614 |
| <I/σ(I)> | 9.27 | 2.22 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.5 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | lithium sulfate |
