5X9V
Crystal structure of group III chaperonin in the Closed state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 186.166, 186.166, 160.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.152 - 3.003 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kfe |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.220 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 56297 |
| <I/σ(I)> | 24.7 |
| Completeness [%] | 99.1 |
| Redundancy | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 295 | 3 mM AMP-PNP, 20 mM MgCl2, 100 mM HEPES NaOH (pH 7.5), 22% Polyacrylic Acid 5100 |
