5X9U

Crystal structure of group III chaperonin in the open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.10000
Spacegroup name	P 4 21 2
Unit cell lengths	209.780, 209.780, 169.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.446 - 4.001
R-factor	0.2563
Rwork	0.253
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x9v 3kfe
RMSD bond length	0.005
RMSD bond angle	1.029
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	4.000
Number of reflections	32570
<I/σ(I)>	8.4
Completeness [%]	99.7
Redundancy	20.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	3 mM ADP, 200 mM NH4Cl, 10mM MgCl2, 50mM HEPES sodium (pH 7.0), 5% w/v Polyethylene glycol PEG 8000, 3% 1,8-Diaminooctane