5X9K

Structure of AusH from Aspergillus nidulans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	50.589, 37.423, 74.777
Unit cell angles	90.00, 107.45, 90.00

Refinement procedure

Resolution	47.055 - 1.798
R-factor	0.2004
Rwork	0.196
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wqf
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.798	1.798
Rmerge	0.058	0.617
Number of reflections	25080	3956
<I/σ(I)>	14.19	1.85
Completeness [%]	99.5	98.4
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Imidazole (pH 8.0), 10% PEG8000, 100 mM Ca(OAc)2