5X9K
Structure of AusH from Aspergillus nidulans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.589, 37.423, 74.777 |
| Unit cell angles | 90.00, 107.45, 90.00 |
Refinement procedure
| Resolution | 47.055 - 1.798 |
| R-factor | 0.2004 |
| Rwork | 0.196 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wqf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.882 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.798 | 1.798 |
| Rmerge | 0.058 | 0.617 |
| Number of reflections | 25080 | 3956 |
| <I/σ(I)> | 14.19 | 1.85 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Imidazole (pH 8.0), 10% PEG8000, 100 mM Ca(OAc)2 |
