5X9J
Structure of PrhC from Penicillium brasilianum NBRC 6234
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 65 |
| Unit cell lengths | 157.064, 157.064, 49.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.695 - 2.100 |
| R-factor | 0.1862 |
| Rwork | 0.185 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wqf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.801 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.074 | 0.562 |
| Number of reflections | 41556 | 6597 |
| <I/σ(I)> | 22.19 | 4.35 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM HEPES (pH 7.5), 1.125M Li2SO4 |
