5X9C
Crystal structure of the cytosolic domain of human MiD51
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.297, 64.666, 65.914 |
| Unit cell angles | 89.81, 108.08, 117.19 |
Refinement procedure
| Resolution | 48.540 - 1.850 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x9b |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.540 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.860 |
| Rmerge | 0.654 | |
| Number of reflections | 68988 | |
| <I/σ(I)> | 23.9 | 5.4 |
| Completeness [%] | 95.3 | 96.5 |
| Redundancy | 5.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.6 M NaH2PO4/K2HPO4, pH 7.0 |
