5X9B
Crystal structure of the cytosolic domain of human MiD51
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.836, 88.836, 124.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.420 - 2.700 |
| R-factor | 0.22585 |
| Rwork | 0.222 |
| R-free | 0.29808 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.420 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.945 | |
| Number of reflections | 14284 | |
| <I/σ(I)> | 15.9 | 2.109 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2 M L-Proline, 0.1 M HEPES pH 7.0, 6% PEG3350 |
