5X7V
Crystal structure of Nucleosome assembly protein S (PfNapS) from Plasmodium falciparum
Replaces: 3KYPExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.790, 114.900, 139.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.244 - 2.802 |
R-factor | 0.2467 |
Rwork | 0.243 |
R-free | 0.31190 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.452 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 35024 | |
<I/σ(I)> | 23.5 | |
Completeness [%] | 96.6 | 81.8 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 20% PEG 3350, 0.2M di-ammonuium tatrate, VAPOR DIFFUSION, HANGING DROP |