5X7M
Crystal structure of meso-diaminopimelate decarboxylase (DAPDC) from Corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.650, 92.013, 94.753 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.708 - 2.400 |
R-factor | 0.1897 |
Rwork | 0.186 |
R-free | 0.25310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hkv |
RMSD bond length | 0.014 |
RMSD bond angle | 1.725 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.760 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 36222 | |
<I/σ(I)> | 26.88 | |
Completeness [%] | 96.0 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Sodium citrate tribasic, Sodium cacodylate |