5X7L
Structure of TsrD from Streptomyces laurentii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2016-04-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 54.232, 54.232, 199.952 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.420 - 1.220 |
R-factor | 0.1514 |
Rwork | 0.151 |
R-free | 0.16631 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.978 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.220 |
Number of reflections | 102592 |
<I/σ(I)> | 38.3 |
Completeness [%] | 99.6 |
Redundancy | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, and 30% v/v 2-Propanol |