5X6X

Crystal structure of the capping enzyme P5 from Rice Dwarf Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-04
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 1
Unit cell lengths	77.230, 83.854, 143.250
Unit cell angles	103.41, 102.47, 95.37

Refinement procedure

Resolution	44.504 - 2.100
R-factor	0.1908
Rwork	0.188
R-free	0.23740
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.822
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.073	0.550
Rpim	0.042	0.320
Number of reflections	194125	9600
<I/σ(I)>	24.5	3.2
Completeness [%]	98.5	97.7
Redundancy	4	4
CC(1/2)		0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	16% PEG 3350, 0.1M MES, pH 6.0