5X6J
Crystal structure of B. globisporus adenylate kinase variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.106, 65.106, 94.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.19679 |
| Rwork | 0.193 |
| R-free | 0.27611 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3g |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.043 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.508 | |
| Number of reflections | 13990 | |
| <I/σ(I)> | 53.98 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 21.6 | 21.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 2.5M ammonium sulfate, 1% polyethylene glycol 1000, 0.1% Sodium azide, 50mM HEPES pH 7.0 |
