Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 92.867, 92.867, 83.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.434 - 3.100 |
| R-factor | 0.2032 |
| Rwork | 0.202 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kmp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.228 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.062 | 0.449 |
| Rpim | 0.025 | 0.173 |
| Number of reflections | 4102 | 402 |
| <I/σ(I)> | 25 | 4.28 |
| Completeness [%] | 97.4 | 100 |
| Redundancy | 7.2 | 7.5 |
| CC(1/2) | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1M Tris, pH 7.0, 12% PEG 3350, 0.2M KNO3 |
