5X5Y

A membrane protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	69.952, 159.868, 166.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.641 - 3.465
R-factor	0.271
Rwork	0.269
R-free	0.30390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qc2
RMSD bond length	0.002
RMSD bond angle	0.652
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.460	7.530	3.500
Rmerge	0.152	0.068	1.930
Rmeas	0.159	0.071	2.028
Rpim	0.043	0.020	0.610
Number of reflections	24569
<I/σ(I)>	5.6
Completeness [%]	99.4	99.2	97.5
Redundancy	13.3	12.7	10.3
CC(1/2)		0.998	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277.15	PEG 2000, Hepes, magnesium chloride, HEGA-10