5X5W

Crystal structure of pseudorabies virus glycoprotein D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	79.945, 98.110, 128.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.166 - 2.700
R-factor	0.2393
Rwork	0.238
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u82
RMSD bond length	0.015
RMSD bond angle	1.512
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rpim		0.419
Number of reflections	28419
<I/σ(I)>	9.6
Completeness [%]	99.9
Redundancy	8.6
CC(1/2)		0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2M sodium chloride, 0.1M sodium acetate pH 5.5, 20% w/v PEG 10000