5X5T
Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (KGSADH) from Azospirillum brasilense
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 129.040, 129.040, 118.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.606 - 2.250 |
R-factor | 0.1777 |
Rwork | 0.174 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w8r |
RMSD bond length | 0.016 |
RMSD bond angle | 1.774 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.100 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 43134 | |
<I/σ(I)> | 27.63 | |
Completeness [%] | 95.1 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 16% polyethylene glycol 3350, 0.1 M sodium cacodylate pH 6.5, and 0.2 M Magnesium chloride hexahydrate |