5X5J
Crystal structure of response regulator AdeR receiver domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.258, 65.258, 50.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.582 - 1.401 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.404 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.580 |
| High resolution limit [Å] | 1.400 |
| Rmerge | 0.115 |
| Number of reflections | 24660 |
| <I/σ(I)> | 15.2 |
| Completeness [%] | 99.8 |
| Redundancy | 19.1 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1M sodium acetate trihydrate pH 4.6, 2.0M sodium formate |
