5X5H

Crystal structure of metB from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	F 2 2 2
Unit cell lengths	58.574, 149.851, 161.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.930 - 1.510
R-factor	0.149
Rwork	0.148
R-free	0.17230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qhx
RMSD bond length	0.027
RMSD bond angle	2.465
Data reduction software	HKL
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.930
High resolution limit [Å]	1.510	1.510
Rmerge	0.074	0.321
Number of reflections	55876
<I/σ(I)>	42.7	16
Completeness [%]	99.3	98.6
Redundancy	6.6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Magnesium formate