5X51
RNA Polymerase II from Komagataella Pastoris (Type-3 crystal)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-28 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 148.770, 158.050, 254.960 |
Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
Resolution | 33.267 - 6.996 |
R-factor | 0.3372 |
Rwork | 0.338 |
R-free | 0.33510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.819 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2614) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.267 | 33.267 | 7.410 |
High resolution limit [Å] | 7.000 | 19.490 | 7.000 |
Rmerge | 0.265 | 0.069 | 1.146 |
Rmeas | 0.309 | 0.081 | 1.335 |
Total number of observations | 70891 | ||
Number of reflections | 18745 | 839 | 2925 |
<I/σ(I)> | 6.3 | 16.22 | 1.54 |
Completeness [%] | 99.1 | 91 | 98.2 |
Redundancy | 3.782 | 3.426 | 3.777 |
CC(1/2) | 0.962 | 0.994 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M sodium citrate, 10% PGA-LM, 0.5% dextran sulfate sodium salt, 15 mM Tris, and 35 mM Hepes |