5X4J
The crystal structure of Pyrococcus furiosus RecJ (Zn-soaking)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.614, 67.347, 60.511 |
Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
Resolution | 39.890 - 2.040 |
R-factor | 0.1787 |
Rwork | 0.177 |
R-free | 0.21170 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.432 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 45535 | |
<I/σ(I)> | 24.8 | 13.5 |
Completeness [%] | 94.1 | 85.4 |
Redundancy | 7.3 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | 4.3% w/v PEG 2000 MME, 50 mM Bicine pH 8.8, 28.6% w/v PEG 600 |