5X42

Structure of DotL(590-659)-DotN derived from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.2828
Spacegroup name	P 21 21 21
Unit cell lengths	50.683, 72.220, 170.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.580 - 1.800
R-factor	0.2121
Rwork	0.211
R-free	0.25980
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.840
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.064	0.048	0.272
Rmeas	0.067	0.050	0.313
Rpim	0.019	0.014	0.149
Number of reflections	104008
<I/σ(I)>	14.9
Completeness [%]	97.0	98.6	91.2
Redundancy	8.8	12.7	3.4
CC(1/2)		0.998	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	100mM sodium malonate (pH 4.0), 10%(v/v) PEG3350, 20%(v/v) glycerol