5X3M
crystal structure of p-Ub-S65-NH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-09 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.914, 29.969, 39.726 |
| Unit cell angles | 81.91, 72.38, 62.43 |
Refinement procedure
| Resolution | 26.565 - 1.818 |
| R-factor | 0.2096 |
| Rwork | 0.206 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.565 |
| High resolution limit [Å] | 1.818 |
| Number of reflections | 9443 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 82.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Lithium Sulfate monohydrate, 0.1M HEPES pH 7.5, 25%(w/v) Polyethylene Glycol 3350 |
