5X3E
kinesin 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 6 |
| Unit cell lengths | 244.152, 244.152, 42.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.845 - 2.610 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.23970 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.736 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.845 | 2.700 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 44542 | |
| <I/σ(I)> | 13.3 | 1.05 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.5M (NH4)2SO4, 100mM MES, 200mM NaI, 4% dioxane, 10mM DTT, pH 6.5 |
