5X3D
Crystal structure of HEP-CMP-bound form of cytidylyltransferase (CyTase) domain of Fom1 from Streptomyces wedmorensis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97908, 0.97927, 0.96404 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 71.909, 71.909, 109.507 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.050 - 1.930 |
| R-factor | 0.19307 |
| Rwork | 0.191 |
| R-free | 0.23376 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.930 |
| Number of reflections | 13233 |
| <I/σ(I)> | 63.1 |
| Completeness [%] | 99.9 |
| Redundancy | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 4.3 M sodium chloride |
