5X32
Crystal structure of D-mannose isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 98.915, 100.929, 192.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.991 - 2.586 |
R-factor | 0.1971 |
Rwork | 0.194 |
R-free | 0.25960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gt5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.231 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.586 | 2.600 |
Number of reflections | 30332 | 4683 |
<I/σ(I)> | 10 | 2.6 |
Completeness [%] | 99.3 | 96.5 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | PEG 3350, Sodium Potassium Phosphate, Bis Tris Propane |