5X2W
Crystal structure of Pseudomonas putida methionine gamma-lyase wild type with L-methionine intermediates
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-03-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 154.635, 152.311, 80.487 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.19797 |
Rwork | 0.195 |
R-free | 0.24635 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o7c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.324 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.169 | 0.435 |
Number of reflections | 53025 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Na-K phosphate buffer, 6-10 % PEG 6000, 0.25 M ammonium sulfate. 0.5 mM PLP |